Studies on the mechanism of hydroxymethylbilane synthase concerning the role of arginine residues in substrate binding
- 15 April 1991
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 275 (2) , 447-452
- https://doi.org/10.1042/bj2750447
Abstract
The role of conserved arginine residues in hydroxymethylbilane synthase was investigated by replacing these residues in the enzyme from Escherichia coli with leucine residues by using site-directed mutagenesis. The kinetic parameters for these mutant enzymes and studies on the formation of intermediate enzyme-substrate complexes indicate that several of these arginine residues are involved in binding the carboxylate side chains of the pyrromethane cofactor and the growing oligopyrrole chain.Keywords
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