Effect of Some of the B Complex Vitamins upon Chick Tissue Cultures

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Abstract
Brain, skin and heart tissues were cultivated by the hanging drop method in a medium of equal parts of chicken plasma and Ringer-Tyrode''s soln. to which riboflavin, nicotinamide, thiamine chloride, pyridoxine, and Ca pantothenate were added separately in concns. of 62.5-2000 [gamma]/ml. of media. Folic acid and biotin were used in concns. of 0.112 and 0.156-3.6 and 5 7 respectively /ml. of medium. High concns. of the vits. were either toxic or sup-pressive; in lesser concns. the vit. cultures grew as well as or insignificantly better than the controls except for cardiac tissue which was not stimulated at all in the presence of thiamine chloride in any of the dilutions used, and was only slightly stimulated by the remaining vits. Brain and skin were stimulated only insignificantly. Most of the vits. did, however, prolong the life of the cultures even though growth was static. Folic acid and biotin were particularly effective in this respect on brain cultures.

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