Cloning of thyroid hormone receptor genes expressed in metamorphosing flounder

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Abstract
Two distinct cDNAs encoding thyroid hormone receptors (THRs) were cloned from a λ gtl0 library prepared from the whole bodies of metamorphosing flounder larvae (Paralichfhys olivaceus). Deduced amino acid sequences of the two isolated cDNAs shared 96% and 92% homologies in their DNA‐ and hormone‐binding domains, respectively. These were highly conserved when compared to THRs for other vertebrates: 88–96% in the DNA‐binding domain and 84–94% in the hormone‐binding domain. Other receptors in the nuclear receptor family showed lower homologies than those of THRs. Both THRs for the flounder had higher homologies with the α‐type THRs of other vertebrates than with the β‐type. Thus, the two THRs for flounder were designated as fTHRαA and fTHRαB. © 1994 Wiiey‐Liss, Inc.