A Venn Diagram Model That Allows Triple-Label Immunophenotypic Analysis of Cells Based upon Double-Label Measurements
- 1 July 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 92 (1) , 73-77
- https://doi.org/10.1093/ajcp/92.1.73
Abstract
Using Venn diagrams derived from set theory, a mathematic model is presented that allows triple-label immunophenotypic analysis of cells based upon double-label measurements. Practical applications of this method are illustrated and discussed. Using this model, triple-label data concerning Leu-8 and Leu-9 (CD7) antigen co-expression by Leu-4+ (CD3+) leukocytes were derived from double-label measurements obtained with a single laser flow cytometer. Mean values were as follows: Leu-4+8+9+ (62%), Leu-4+8−9+ (36%), Leu-4+8+9− (0%), and Leu-4+8−9− (0%). Examples are given of additional models based upon similar principles.This publication has 5 references indexed in Scilit:
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