A simple enzymic method for the synthesis of adenosine 5′-[α-32P]triphosphate on a preparative scale
- 1 March 1977
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 161 (3) , 555-559
- https://doi.org/10.1042/bj1610555
Abstract
A simple, rapid and inexpensive method is described for the enzymic synthesis of [alpha-32P]ATP from [32P]Pi on a preparative scale with an overall yield of 53%. The final product contained all of the detectable radioactivity (less than 99.9%) in the alpha position and has been shown to behave identically with commerically availabe [alpha-32P]ATP during the synthesis of 3′:5′-cyclic AMP in the reaction catalysed by adenylate cyclase.This publication has 5 references indexed in Scilit:
- Modified procedure for the synthesis of 32P-labelled ribonucleoside 5′-monophosphates of high specific activityBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1968
- Some properties of adenosine kinase from Ehrlich ascites-tumour cellsBiochemical Journal, 1967
- A simple method for the preparation of 32P-labelled adenosine triphosphate of high specific activityBiochemical Journal, 1964
- A spectrophotometric method for the determination of creative phosphokinase and myokinaseBiochemical Journal, 1955
- ENZYMATIC PHOSPHORYLATION OF ADENOSINE AND 2,6-DIAMINOPURINE RIBOSIDEJournal of Biological Chemistry, 1951