Mechanism for interference of carbamazepine in Porter-Silber reaction for determination of urinary 17-hydroxycorticosteroids.

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Abstract
The mechanism by which dosage of carbamazepine (5H-dibenz [b, f] azepine-5-carboxamide) interferes with the Porter-Silber reaction for the determination of urinary 17-hydroxycorticosteroids has been investigated. A specimen of human urine collected following oral administration of carbamazepine was processed in the usual manner. The principal metabolites responsible for the Porter-Silber test were separated and characterized to be carbamazepine-10, 11-epoxide and dihydrocarbamazepine-10, 11-trans-diol by usual criteria. Being treated with the incomplete Porter-Silber reagent, these metabolites underwent facile rearrangement yielding acridine-9-aldehyde accompanied with a small amount of acridine. It has been disclosed that the interference in the determination of 17-hydroxycorticosteroids due to the undesirable coloration is ascribable to the formation of acridine-9-aldehyde phenylhydrazone from the drug metabolites.

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