Association of N‐ethylmaleimide‐sensitive factor with synaptic vesicles
- 22 August 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 350 (2-3) , 253-257
- https://doi.org/10.1016/0014-5793(94)00778-0
Abstract
N‐Ethylmaleimide‐sensitive factor (NSF) mediates docking and/or fusion of transport vesicles in the multi‐pathways of vesicular transport. NSF was highly expressed in brain and adrenal gland. Immunostaining of cerebellum with an anti‐NSF monoclonal antibody showed that NSF is predominantly localized in the molecular layers and the glomeruli of the granule cell layers. This distribution coincided well with that of synaptophysin, a marker protein of synaptic vesicles. Purification and immunoprecipitation revealed that NSF is associated with brain synaptic vesicles. The present results suggest that NSF is associated with synaptic vesicles without Ca2+ influx.Keywords
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