The Effect of Cyclic Amp on the Accumulation of Newly Synthesized Protein in the Extracellular Matrix of Pfhr-9 Teratocarcinoma Cells
- 1 January 1987
- journal article
- research article
- Published by Taylor & Francis in Connective Tissue Research
- Vol. 16 (1) , 95-107
- https://doi.org/10.3109/03008208709001997
Abstract
The effect of elevated cyclic AMP levels on the accumulation of newly synthesized extracellular matrix protein was examined in PFHR-9 cells producing Type IV collagen. The effect of dbcAMP, 8-BrcAMP, IBMX and forskolin on the synthesis of total protein, non-collagen protein and collagen were compared. DbcAMP increased the accumulation of total protein but did not affect the distribution of collagen and non-collagen protein. 8-BrcAMP, IBMX and forskolin also increased collagen and non-collagen accumulation. However, the effect on collagen was significantly greater with 8-BrcAMP and IBMX. Consequently, 8-BrcAMP and IBMX resulted in an increased percent collagen synthesis in the extracellular matrix. Elevated cAMP levels had no effect on cell proliferation or DNA synthesis but did produce a significant effect on cell morphology.Keywords
This publication has 39 references indexed in Scilit:
- Induction of reverse transformation and normal cell cycle regulation by dibutyryl cAMP in a chemically transformed cell lineJournal of Cellular Physiology, 1983
- Characterization of the collagens synthesized by Chinese hamster ovary cells: effect of colcemid and dibutyryl adenosine cyclic monophosphateBiochemistry, 1982
- Modulation of collagen production by fibroblasts. Effects of chronic exposure to agonists that increase intracellular cyclic AMPBiochemical Journal, 1982
- Acute effects of gonadotrophins and cyclic AMP on protein synthesis and progesterone production by isolated rat granulosa cellsActa Physiologica Scandinavica, 1981
- Evidence for acceleration of the rate of elongation of tyrosine aminotransferase nascent chains by dibutyryl cyclic AMPProceedings of the National Academy of Sciences, 1978
- Effect of Dibutyryl-Cyclic AMP on Collagen and Non-Collagen Protein Synthesis in Cultured Human CellsConnective Tissue Research, 1974
- Regulation of Tyrosine Hydroxylase Activity in Cultured Mouse Neuroblastoma Cells: Elevation Induced by Analogs of Adenosine 3′:5′-Cyclic MonophosphateProceedings of the National Academy of Sciences, 1972
- Further Changes in Differentiation State Accompanying the Conversion of Chinese Hamster Cells to Fibroblastic Form by Dibutyryl Adenosine Cyclic 3′:5′-Monophosphate and HormonesProceedings of the National Academy of Sciences, 1971
- Restoration of Several Morphological Characteristics of Normal Fibroblasts in Sarcoma Cells Treated with Adenosine-3′:5′-Cyclic Monophosphate and Its DerivativesProceedings of the National Academy of Sciences, 1971
- Morphological Transformation of Chinese Hamster Cells by Dibutyryl Adenosine Cyclic 3′:5′-Monophosphate and TestosteroneProceedings of the National Academy of Sciences, 1971