Nereis sarcoplasmic Ca2+‐binding protein has a highly unstructured apo state which is switched to the native state upon binding of the first Ca2+ ion
- 14 October 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 395 (1) , 89-94
- https://doi.org/10.1016/0014-5793(96)01007-1
Abstract
NSCP, a sarcoplasmic Ca2+/Mg2+-binding protein from Nereis diversicolor, shows an allosteric change during Ca2+ binding and a high positive cooperativity for Mg2+ binding. Here we report the results of CD and NMR experiments aiming to characterize the apo state and the Ca2+-induced conformational changes in this protein. Circular dichroism spectra of the apo form are indicative of a reduced helical structure. In contrast, NMR spectra show no element of regular secondary or tertiary structure. Addition of one Ca2+ determines large spectral changes bringing the molecule in a conformation which is very close to the native three Ca 2+ state. Addition of the second and third Ca 2+ shifts this equilibrium progressively towards the liganded conformation but affects only minimally the spectrum of the liganded species.Keywords
This publication has 38 references indexed in Scilit:
- Extensive nonrandom structure in reduced and unfolded bovine pancreatic trypsin inhibitorBiochemistry, 1995
- Solution Structure of (Cd2+)1-calbindin D9kReveals Details of the Stepwise Structural Changes along the Apo*rarr;(Ca2+)1II*rarr;(Ca2+)2I,IIBinding PathwayJournal of Molecular Biology, 1995
- Calcium-induced conformational transition revealed by the solution structure of apo calmodulinNature Structural & Molecular Biology, 1995
- Structures of folding intermediatesCurrent Opinion in Structural Biology, 1995
- Quantification of the calcium‐induced secondary structural changes in the regulatory domain of troponin‐CProtein Science, 1994
- Protein Folding: Solid evidence for molten globulesCurrent Biology, 1994
- Structure and dynamics of the acid-denatured molten globule state of .alpha.-lactalbumin: a two-dimensional NMR studyBiochemistry, 1993
- Thermodynamics of cation binding to Nereis sarcoplasmic calcium‐binding proteinEuropean Journal of Biochemistry, 1992
- Structure of a sarcoplasmic calcium-binding protein from Nereis diversicolor refined at 2·0 Å resolutionJournal of Molecular Biology, 1992
- Complete assignment of the 1H NMR spectrum of a synthetic zinc finger from Xfin Sequential resonance assignments and secondary structureFEBS Letters, 1989