A COMPARISON OF THE HISTAMINE-RELEASING PROPERTIES OF RAT PLEURAL AND PERITONEAL MAST-CELLS

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  • 1 January 1980
    • journal article
    • research article
    • Vol. 41  (2) , 271-278
Abstract
A comparison was made of the histamine-releasing characteristics of rat mast cells of pleural and peritoneal origin in response to a wide variety of immunological and non-immunological stimuli, i.e., IgE antibody-antigen interaction; rabbit anti-rat IgE antibody; concanavalin (Con) A, ACTH (1-24) polypeptide (Synacthen); a decapeptide comprising an amino acid sequence (497-506) within the human .epsilon.-chain (Stanworth, et al., 1979); ATP and the Ca ionophore A 23187 (calcimycin). This has provided valuable information about the relative responsiveness of target cells from 2 different sources within the same species. Peritoneal cells proved to be more responsive to basic polypeptide liberators, whereas pleural cells were considerably more responsive to stimuli mediated through IgE antibody, and slightly more responsive to challenge with non-immunological liberators. Interestingly, the histamine-release studies using an antiserum raised against mast cell IgE receptors have indicated that pleural mast cell membranes contain a higher density of IgE receptors than peritoneal mast cell membranes. The amount of histamine released from peritoneal or pleural mast cells effected by the polypeptide liberators was not influenced by the prior occupancy of the mast cell Fc receptors by rat IgE antibody.