Regulation of Ca‐sensitive inactivation of a l‐type Ca2+ channel by specific domains of β subunits
- 30 April 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 450 (1-2) , 17-22
- https://doi.org/10.1016/s0014-5793(99)00463-9
Abstract
Ca2+ channel auxiliary β subunits have been shown to modulate voltage‐dependent inactivation of various types of Ca2+ channels. The β1 and β2 subunits, that are differentially expressed with the L‐type α1 Ca2+ channel subunit in heart, muscle and brain, can specifically modulate the Ca2+‐dependent inactivation kinetics. Their expression in Xenopus oocytes with the α1C subunit leads, in both cases, to biphasic Ca2+ current decays, the second phase being markedly slowed by expression of the β2 subunit. Using a series of β subunit deletion mutants and chimeric constructs of β1 and β2 subunits, we show that the inhibitory site located on the amino‐terminal region of the β2a subunit is the major element of this regulation. These results thus suggest that different splice variants of the β2 subunit can modulate, in a specific way, the Ca2+ entry through L‐type Ca2+ channels in different brain or heart regions.Keywords
This publication has 35 references indexed in Scilit:
- Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extensionPublished by Elsevier ,2003
- Transfer of 1,4-Dihydropyridine Sensitivity from L-Type to Class A (BI) Calcium ChannelsNeuron, 1996
- Ca2+‐dependent inactivation of the class C L‐type Ca2+ channel is a property of the α1 subunitFEBS Letters, 1996
- Essential Ca 2+ -Binding Motif for Ca 2+ -Sensitive Inactivation of L-Type Ca 2+ ChannelsScience, 1995
- Ca2+ channel regulation by a conserved β subunit domainNeuron, 1994
- Calcium currents recorded from a neuronal α1C L‐type calcium channel in Xenopus oocytesFEBS Letters, 1994
- Calcium channel β-subunit binds to a conserved motif in the I–II cytoplasmic linker of the α1-subunitNature, 1994
- Molecular localization of regions in the L-type calcium channel critical for dihydropyridine actionNeuron, 1993
- Cloning and tissue‐specific expression of the brain calcium channel β‐subunitFEBS Letters, 1991
- Characterization of cDNA clones encoding two putative isoforms of the α1 subunit of the dihydropyridine‐sensitive voltage‐dependent calcium channel isolated from rat brain and rat aortaFEBS Letters, 1989