Isolation and amino acid sequence of the smallest subunit of beef heart bc1 complex
- 7 October 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 190 (1) , 89-94
- https://doi.org/10.1016/0014-5793(85)80434-8
Abstract
The 11 subunits of beef heart bc 1 complex can be separated either by a new SDS-PAGE system or by a series of Chromatographic steps involving dissociation of the complex by salt treatment. The amino acid sequence of the smallest subunit was determined by complete solid-phase Edman degradation and was confirmed by sequencing the N-terminal part and the C-terminal tryptic fragment by liquid-phase Edman degradation. The protein consists of 56 amino acid residues; the M r was calculated to be 6363. The protein (‘ISP binding factor’) might be entangled in the reassembly of the iron-sulfur protein with the bc 1 subcomplex.Keywords
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