Blood Histamine, Leukocytes and Platelets in Experimental Serum Disease in Rabbits

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Abstract
Serum disease was produced in 40 rabbits by 2 intraven. injns. of 15 cc. per kg. of horse serum each. 16 of these rabbits received 20 mg. of neohetramine in a 4% soln. subcut. 4 times daily. Histamine was detd. according to Code''s method. The first injn. of serum alone was followed by a rise in histamine and platelets with a peak on the 4th day, the 2d by a drop in these elements. There was a rise in eosinophiles (on the 1st day after 1st injn.), followed by a rise in neutrophiles (between the 2d and 4th day) and lymphocytes (on the 4th day), and finally a moderate rise in basophiles (during the 2d and 3d week). Following the 2d injn. of serum, the lymphocytes and basophiles showed a marked drop (on the 1st day), and this was followed by a marked rise of the 2 with a peak on the 4th day. The rise in basophiles averaged 1300%. The latter was interpreted as a regenerative phenomenon prompted by the heparin-consuming effect of the anaphylactic reaction. Neohetramine was found to effect only the primary reactions following the 1st admn. of serum. It suppressed the rise in blood histamine and the proteinuria observed during the 1st week following the 1st injn., and it also impeded the primary mesenchymal reactions in lungs and heart which are believed to be instrumental in antibody formation. There is reason to believe that this was not a specific antihistaminic effect, but that it was due to a non-specific action of the drug upon the permeability of tissue membranes possibly via the hyaluronidase-hyaluronic acid mechanism.

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