Further Analysis of Poly-L-lysine Chromatogram on Hydroxyapatite Column
- 1 May 1982
- journal article
- research article
- Published by Taylor & Francis in Separation Science and Technology
- Vol. 17 (6) , 785-795
- https://doi.org/10.1080/01496398208058322
Abstract
Earlier the multipeak chromatogram of low-molecular weight poly-L-lysine obtained by Bernardi on a hydroxyapatite column was analyzed on the basis of a chromatographic theory. Chromatography was carried out in the presence of a linear molarity gradient of sodium phosphate buffer at pH 6.8. In the present paper, on the basis of a recently developed new theory, a peak in the multipeak chromatogram is analyzed again in detail. By taking into account an entropy effect occurring due to the variation in number of geometrical configurations of a molecule adsorbed on the crystal surface of hydroxyapatite, the width in the experimental peak can be satisfactorily explained.This publication has 18 references indexed in Scilit:
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