Diluters for Bovine Semen. III. Effect of Lactenin and of Lactoperoxidase upon Spermatozoan Livability

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Abstract
Lactenin, an antistreptococcal substance of milk, was concentrated by acetone fractionation of whey and shown to be highly toxic to bovine spermatozoa. Lactenin prepared by tryptic digestion of whey, dialysis and alcoholic precipitation also was toxic to spermatozoa but the results were partially confounded by the difficulty of separating trypsin from the lactenin. Lactoperoxidase, when added to heated skim milk and used as a semen diluent in a storage trial, exhibited no toxicity for bovine spermatozoa at the concentration found in milk.
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