Biotechnological production of D‐ or L‐amino acids from 5‐monosubstituted hydantoins
- 1 January 1990
- journal article
- other
- Published by Taylor & Francis in Food Biotechnology
- Vol. 4 (1) , 87-95
- https://doi.org/10.1080/08905439009549725
Abstract
The great advantage of producing optically pure D‐ or L‐amino acids from D,L‐5‐monosubstituted hydantoins by bioconversion is the fact that most of the substrates already racemize under reaction conditions and are totally converted to the optically pure amino acids. Three enzymes were shown to be involved in the production of L‐tryptophan from D,L‐5‐indolylmethylhydantoin in Arthrobacter spec. DSM 3747 after growth in a medium containing the chemically synthesized D.L‐5‐indolyl‐3‐N‐methlylhydantoin as effective inducer. Besides a hydantoinase and a N‐carbamoyl‐L‐amino acid amidohydrolase, a 5‐indolylmethylhydantoin racemase was found and could be separated from the former enzymes by liquid‐liquid extraction and gel filtration.Keywords
This publication has 8 references indexed in Scilit:
- Production of D- and L-Amino Acids from D, L-5-Monosubstituted HydantoinsAnnals of the New York Academy of Sciences, 1988
- Stereo- and substrate-specificity of a d-hydantoinase and a
d carbamyl-amino acid amidohydrolase of Arthrobacter crystallopoietes AM 2Enzyme and Microbial Technology, 1988 - Preparation of D-valine from D,L-5-isopropylhydantoin by stereoselective biocatalysisJournal of Molecular Catalysis, 1988
- Enzymatic production of l-tryptophan from dl-5-indolylmethylhydantoin by Flavobacterium sp.Enzyme and Microbial Technology, 1987
- Mechanism of Asymmetric Production of l-Aromatic Amino Acids from the Corresponding Hydantoins by Flavobacterium sp.Agricultural and Biological Chemistry, 1987
- Substrate- and stereospecificity, induction and metallo-dependence of a microbial hydantoinaseBiotechnology Letters, 1987
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Production ofl-Glutamic Acid fromdl-Hydantoin-5-propionic Acid by MicrooganismsAgricultural and Biological Chemistry, 1966