Uptake of β-Hexosaminidase by Nonparenchymal Liver Cells and Peritoneal Macrophages

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Abstract
The uptake of β-hexosaminidase (EC 3.2.1.30) in nonparenchymal liver cells (i.e. endothelial and Kupffer’s cells) and peritoneal macrophages has been determined by an enzymatic assay. A considerable uptake was noted in nonparenchymal liver cells, whereas no measurable uptake was seen in peritoneal macrophages. The endothelial cells were more active in the uptake of β-hexosaminidase than were the Kupffer’s cells. The uptake of β-hexosaminidase by nonparenchymal liver cells showed saturation kinetics and was competetively inhibited by mannan. These findings support the concept that a cell surface receptor on nonparenchymal liver cells mediates uptake of β-hexosaminidase and suggests a difference in the receptor mechanisms on liver and peritoneal macrophages.