A Technique for Obtaining Uniform Inocula of Actinomyces israelii

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Abstract
The anaerobic filamentous organism Actinomyces israelii grows as discrete colonies which sediment in static liquid media and are too large and variable in size to be suitable for quantitative inoculations of experimental media. A stirred-culture technique yielded uniformly small viable colonies of Actinomyces israelii. The microcolonies obtained still sedimented rapidly but could be kept in even suspension by bubbling nitrogen through the suspension. Such suspensions gave uniform inocula from a graduated Pasteur pipette as Judged by total-nitrogen determinations and showed the growth characteristics of A. israelii grown in static liquid media.