Nucleic Acid-binding Properties of Adenovirus Structural Polypeptides
Open Access
- 1 November 1982
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 63 (1) , 69-79
- https://doi.org/10.1099/0022-1317-63-1-69
Abstract
The ability of adenovirus structural polypeptides to bind nucleic acids was assessed by separating the polypeptides on sodium dodecyl sulfate-polyacrylamide gels, transferring them electrophoretically to nitrocellulose and probing with 32P-labeled nucleic acids. Polypeptides IVa2, V, VI and VII, as well as trace amounts of pVII and a polypeptide of apparent MW 40 .times. 103 were able to bind label under these conditions. Labeling was also detected with a smaller polypeptide, possibly related to the cleavage products of pVII and/or pVI. The binding of DNA to polypeptide VI appeared to be more sensitive to detergents than the others. No sequence specificity could be detected in the DNA binding.This publication has 18 references indexed in Scilit:
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