STIMULATION OF THYMUS-DERIVED AND BONE MARROW-DERIVED LYMPHOCYTES BY TUMOR-CELLS IN CULTURE

  • 1 January 1977
    • journal article
    • research article
    • Vol. 37  (2) , 603-609
Abstract
In vitro lymphocyte stimulation by mitomycin-blocked [mouse] tumor cells can be used to measure tumor-specific immune responses. In order to determine the responding cell type(s) in this reaction, lymph node and spleen cell populations were specifically depleted of thymus- [T] or bone marrow-derived [B] cells by the use of the appropriate [rabbit] antisera and complement or by immunoadsorption of the Fc receptor-bearing cells to antibody-coated sheep red blood cell monolayers. The compositions of the original and the modified lymphocyte populations were determined by viability counting following treatment with antisera and complement, direct and indirect immunofluorescence, antibody-coated erythrocyte rosette formation and respone to T and B cell mitogens. In the lymph node cell populations, only the T cells were stimulated by the tumor cells. However, B and T cells from tumor-immune spleens underwent stimulation when exposed to tumor cells in culture.

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