Use of Percolation for the Extraction of Vasopressin1
- 1 January 1962
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 70 (1) , 43-46
- https://doi.org/10.1210/endo-70-1-43
Abstract
Beef posterior pituitary powder was percolated with ethanol containing increasing concentrations of water and of acetic acid. The percolate with 70% ethanol containing 0.05 M acetic acid was rich in vasopressin. Further purification of vasopressin was effected by electrophoresis in 0.5 M acetic acid at 1000 volts. The best preparation of vasopressin, produced by a combination of percolation and electrophoresis, had a potency of 450 units per mg. Acid hydrolysis of this preparation yielded only the amino acids of arginine vasopressin.Keywords
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