CONCENTRATION OF CORTICOTROPHIN BY PERCOLATION

Abstract
Percolation of commercial anterior pituitary powder with decreasing concentrations of ethanol (95 → 70%) and increasing concentrations of acetic acid (0 → 0.5 M) yielded a peak of corticotrophin activity with 70% ethanol containing 0.5 M acetic acid. The material assayed at about 14 units/mg peptide. Further purification by curtain electrophoresis yielded a preparation with 21 units/mg peptide.