Hoechst 33258 as a vital stain for plant cell protoplasts
- 1 December 1981
- journal article
- Published by Springer Nature in Plant Cell Reports
- Vol. 1 (2) , 77-79
- https://doi.org/10.1007/bf00269279
Abstract
A method for staining nuclei in unfixed protoplasts using Hoechst 33258 is described. It was found that the dye was not taken up by all protoplasts except in the presence of a detergent and that the staining of the nuclei was pH- and concentration-dependent. The use of vital fluorescent probes with plant protoplasts may be helpful for the identification, selection and subsequent culture of hybrid fusion products in automatic cell sorting systems.Keywords
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