Comparison of alkaline phosphatase and horseradish peroxidase conjugated antisera in the ELISA test for antibodies to reovirus 3, mouse hepatitis and Sendai viruses

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Abstract
The method of enzyme-linked immunoadsorbent assay (ELISA) has been applied to the detection of antibodies to reovirus 3, Sendai virus and mouse hepatitis virus (type 1), and the serological response of mice after infection has been followed for 28 days to investigate the earliest appearance of ELISA titres. This has been compared to the appearance of haemagglutination-inhibiting and complement-fixing antibodies. Alkaline phosphatase conjugated antiserum produces the most sensitive and convenient ELISA for the murine viruses examined.