Studies on the amide and C-terminal residues in proteins. 4. Separation and quantitative determination of β-amino alcohols

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Abstract
The quantitative chromatographic separation of [beta]-amino alcohols on partition columns of buffered silica gel, with ethanol-chloroform mixtures as eluting agents, is described. The recoveries vary between 96 and 100%. A method for the quantitative estimation of [beta]-amino alcohols, based on the formaldehyde given on periodate oxidation, was developed. The procedure was used to separate and identify the [beta]-amino alcohols present in the hydrolysates of esterified and reduced proteins. The use of silver oxide to oxidize [beta]-amino alcohols to the corresponding [alpha]a-amino acid is described.