Renin inactivation during in vitro experiments

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Abstract
Experiments were designed to clarify the factors affecting renin released during in vitro experiments. Kidneys from rat, dog and pig were used. Experiments were done in which the gas phase was either bubbled through the incubation medium or layered above it. Renin released into the incubation medium disappeared very rapidly when gas was bubbled through the medium. The decline was similar in mediums bubbled with O2-CO2 (95%-5%) or N-CO2 (95%-5%). The half-life of renin activity in the bubbled medium was approximately 15 min in both cases. In experiments in which nonbubbled medium was used throughout, renin released into the incubation medium did not disappear after removal of slices. The renin released into the incubation medium is probably inactivated at the air-water interface.