Molecular cloning, expression and immunological characterisation of Lol p 5C, a novel allergen isoform of rye grass pollen demonstrating high IgE reactivity1
- 30 November 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 462 (3) , 435-441
- https://doi.org/10.1016/s0014-5793(99)01576-8
Abstract
A novel isoform of a major rye grass pollen allergen Lol p 5 was isolated from a cDNA expression library. The new isoform, Lol p 5C, shares 95% amino acid sequence identity with Lol p 5A. Both isoforms demonstrated shared antigenic activity but different allergenic activities. Recombinant Lol p 5C demonstrated 100% IgE reactivity in 22 rye grass pollen sensitive patients. In comparison, recombinant Lol p 5A showed IgE reactivity in less than 64% of the patients. Therefore, Lol p 5C represents a novel and highly IgE‐reactive isoform allergen of rye grass pollen.Keywords
This publication has 24 references indexed in Scilit:
- Molecular basis of IgE-recognition of Lol p 5, a major allergen of rye-grass pollenMolecular Immunology, 1998
- Localization of antigenic sites on Der p 2 using oligonucleotide-directed mutagenesis targeted to predicted surface residuesClinical and Experimental Allergy, 1997
- Cloning and expression in yeast Pichia pastorisof a biologically active form of Cyn d 1, the major allergen of Bermuda grass pollenJournal of Allergy and Clinical Immunology, 1996
- Cloning, sequencing and expression in Escherichia coli of Pha a 1 and four isoforms of Pha a 5, the major allergens of canary grass pollenClinical and Experimental Allergy, 1995
- Allergen nomenclatureJournal of Allergy and Clinical Immunology, 1995
- Major allergen Phl p Vb in timothy grass is a novel pollen RNaseFEBS Letters, 1995
- Identification of canary grass (Phalaris aquatica) pollen allergens by immunoblotting: IgE and IgG antibody‐binding studiesAllergy, 1993
- Cloning sequencing of Lol pI, the major allergenic protein of rye‐grass pollenFEBS Letters, 1991
- Development of Monoclonal Mouse Antibodies Specific for Allergenic Components in Ryegrass (Lolium perenne) PollenInternational Archives of Allergy and Immunology, 1983
- Aerobiology of Grass Pollen in the City Atmosphere of Melbourne: Effects of Weather Parameters and Pollen Sources.Australian Journal of Botany, 1979