Biological Method for Determination of Glucocorticoid Activity of Crystalline Compounds and Urine Extracts.

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Abstract
A practical bioassay technique for glucocorticoids is proposed, using an adrenalectomized, 21-day old male rat as the test animal. The method offers the accuracy and precision shown by the use of the young adult rat, and the sensitivity and economy observed in methods using the laboratory mouse. Dose-response curves were established for cortisone and for the assay of urine extracts. The regression lines for the 2 substances are parallel, indicating the method is valid for measurement of glucocorticoid excretion. It is suggested that this bioassay method may serve as a valuable adjunct to the chemical methods for the assay of urinary corticoids.