COMPARISON OF LIVER-GLYCOGEN DEPOSITION AND WORK PERFORMANCE TESTS FOR THE BIO-ASSAY OF ADRENAL CORTEX HORMONES

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Abstract
FOR THE BIO-ASSAY of adrenal cortical hormone preparations the survival-growth test (Cartland and Kuizenga, 1936) is of value, since it is responsive to the composite activity of adrenal cortex extracts with respect to both the known compounds and amorphous fractions (Kuizenga, Nelson and Ingle, 1943; and Kuizenga, Nelson, Lyster and Ingle, 1945). Factors influencing electrolyte and carbohydrate metabolism cannot, however, be differentiated by this test. Responses obtained with the pure C-11-oxygenated steroids in the muscle work test, compared with the relative inactivity of desoxycorticosterone and the amorphous fractions by this test, led us to the conclusion that it could be used for the quantitative determination of the specific factors effective in carbohydrate metabolism (Ingle, 1944; and Ingle and Kuizenga, 1945). For clinical use, a knowledge of the content of these factors in adrenal cortical extracts is of importance (MacBryde and De La Baize, 1944; and Forsham, Thorn and Bergner, 1946). We have therefore employed the work test to obtain such information.