Highly sensitive enhanced chemiluminescence immunodetection method for herpes simplex virus type 2 Western immunoblot
- 1 April 1992
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 30 (4) , 1005-1007
- https://doi.org/10.1128/jcm.30.4.1005-1007.1992
Abstract
Three Western blot (immunoblot) methods for detecting antibodies to herpes simplex virus type 2 were compared: (i) nitrocellulose blots with 4-chloro-1-naphthol immunostaining (4CN-WB); (ii) polyvinylidene difluoride (PVDF) blots with 3,3',5,5'-tetramethylbenzidene immunostaining (TMB-WB); and (iii) PVDF blots with enhanced chemiluminescence (ECL-WB). TMB-WB was 10-fold more sensitive than 4CN-WB, while ECL-WB was as much as 500-fold more sensitive.Keywords
This publication has 12 references indexed in Scilit:
- Neonatal Herpes Simplex Virus Infection in Relation to Asymptomatic Maternal Infection at the Time of LaborNew England Journal of Medicine, 1991
- Double-Blind, Placebo-Controlled Trial of a Herpes Simplex Virus Type 2 Glycoprotein Vaccine in Persons at High Risk for Genital Herpes InfectionThe Journal of Infectious Diseases, 1990
- The Frequency of Unrecognized Type 2 Herpes Simplex Virus Infection Among Women Implications for the Control of Genital HerpesSexually Transmitted Diseases, 1990
- Chronic-Dose Acyclovir to Suppress Frequently Recurring Genital Herpes Simplex Virus Infection: Effect on Antibody Response to Herpes Simplex Virus Type 2 ProteinsThe Journal of Infectious Diseases, 1988
- Differential effect of systemic acyclovir treatment of genital HSV-2 infections on antibody responses to individual HSV-2 proteinsJournal of Medical Virology, 1988
- Use of densitometric analysis for interpreting HSV serologies based on Western blotJournal of Virological Methods, 1987
- Antibody response to type-common and type-unique epitopes of herpes simplex virus polypeptidesJournal of Medical Virology, 1985
- Serologic analysis of first-episode nonprimary genital herpes simplex virus infection: Presence of type 2 antibody in acute serum samplesThe American Journal of Medicine, 1984
- The Effects of Acyclovir on Antibody Response to Herpes Simplex Virus in Primary Genital Herpetic InfectionsThe Journal of Infectious Diseases, 1984
- Effect of acyclovir treatment of primary genital herpes on the antibody response to herpes simplex virus.Journal of Clinical Investigation, 1984