QUANTITATIVE LYMPHOSCINTIGRAPHY .1. BASIC CONCEPTS FOR OPTIMAL UPTAKE OF RADIOCOLLOIDS IN THE PARASTERNAL LYMPH-NODES OF RABBITS

  • 1 January 1979
    • journal article
    • research article
    • Vol. 20  (10) , 1038-1046
Abstract
Activity-size distribution of radiocolloids was studied using gel-chromatography scanning (GCS) of columns filled with Sepharose 4B gel. Rabbits were injected s.c. with the colloid of interest, laid supine beneath a .gamma. camera and imaged every 15 s for 2-4 h. From the stored data, uptakes in parasternal lymph nodes were analyzed in terms of 2-compartment model, and the rate constants measured. Substances tested were 198Au colloid, 99mTc antimony sulfide colloid, 99mTc tin colloid, 99mTc phytate and 99mTc sulfur colloid. Optimal particle size for the colloid ranged from 1-10 nm. The largest and most rapid uptake was 198Au colloid, with a particle size of 5 nm, which appeared as a single peak in the GCS spectrum. Percentage uptake after 2 h for 198Au colloid was 8%; it was 5% for antimony-sulfide colloid, which was the best 99mTc-labeled colloid. The GCS spectrum for the antimony product showed a single-peaked size distribution with a somewhat broader range: 5-15 nm. Other colloid particles were too large to pass into the lymphatic system, or too small to be trapped.

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