Restriction and modification in Bacillus subtilis: Identification of a gene in the temperate phage SPβ coding for a BsuR specific modification methyltransferase
- 1 November 1980
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 180 (2) , 361-367
- https://doi.org/10.1007/bf00425849
Abstract
A gene coding for a modifying DNA-methyltransferase which methylates the central C in the BsuR recognition sequence 5′GGCC was identified in the genome of the temperate Bacillus subtilis phage SPβ. This gene is expressed only after induction of the prophage by either mitomycin C or UV. The presence of active methyltransferase in induced cells leads to modification of BsuR recognition sites in SPβ DNA as well as in heterologous DNA.Keywords
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