Transient complementation of xeroderma pigmentosum cells by microinjection of poly(A)+ RNA.

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Abstract
An assay was developed in which excision repair deficiency of xeroderma pigmentosum cells is transiently complemented, as measured by unscheduled DNA synthesis, by microinjection of cytoplasmic poly (A[adenylated])+ RNA derived from HeLa cells. Four different complementation groups of xeroderma pigmentosum were assayed. Groups A and G showed complementation, whereas groups D and F did not. Survival for cells in each of the groups subsequent to microinjection was .apprxeq. 75%. Approximately 10-25% of surviving cells from groups A and G were complemented, as judged by near normal unscheduled DNA synthesis. Fractionation of cytoplasmic poly (A)+ RNA on a 15-30% nondenaturing sucrose gradient and subsequent microinjection of individual fractions indicate that repair mRNA that complement xeroderma pigmentosum groups A and G sediment at .apprx. 11 S and 12 S, respectively. This assay should be of great utility in the cloning and biochemical analysis of DNA repair genes.