In vivotranscription of theE.coli uvrBgene: both promoters are inducible by UV
- 1 January 1983
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (13) , 4355-4363
- https://doi.org/10.1093/nar/11.13.4355
Abstract
The transcriptional activity of the tandem promoters of the Escherichia coli uvrB gene was measured in vivo. Both promoters are shown to be inducible by UV irradiation. P1, the most proximal promoter, is responsible for the main part of transcription both in uninduced and induced cells. Plasmids have been constructed carrying small deletions in the lexA binding site that overlaps with P2, the distal promoter. These deletions result in constitutive transcription from P1. This indicates that the DNA region which contains P2 functions mainly as a target site for regulation of P1 transcription in vivo.Keywords
This publication has 14 references indexed in Scilit:
- Improved estimation of DNA fragment lengths from agarose gelsAnalytical Biochemistry, 1981
- Induction of SOS functions: Regulation of proteolytic activity of E. coil RecA protein by interaction with DNA and nucleoside triphosphateCell, 1981
- Expression of the E. coli uvrA gene is inducibleNature, 1981
- Organization of the lexA gene of Escherichia coli and nucleotide sequence of the regulatory regionNucleic Acids Research, 1981
- Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fdGene, 1980
- DNA Repair in Bacteria and Mammalian CellsAnnual Review of Biochemistry, 1979
- Unusual location and function of the operator in the Escherichia coli galactose operonNature, 1979
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977
- Genetic studies of the lac repressorJournal of Molecular Biology, 1977
- Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli.1976