Hydrolyzed Saliva as a Source of Nutrients Essential for the Production of Acid by an Oral Strain of Lactobacillus Acidophilus

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Abstract
In previous expts. whole saliva was found incapable of supplying amino N in a synthetic medium for the growth of an oral strain of Lactobacillus. In the present expt. whole saliva was hydrolyzed by means of acid and also by Ba(OH)2. When the acid hydrolyzate of saliva was used to replace casein hydrolyzate in the synthetic medium, maximum growth was obtained with an oral strain of Lactobacillus. Comparison of hydrolyzates demonstrated that per unit of amino N the saliva hydrolyzate was more potent than casein hydrolyzate in promoting growth of the organism. The alkaline hydrolyzate of saliva furnished sufficient free tryptophane as well as total amino N for max. growth of the organism.