Conductimetric Assay of a Bacterial Lipase, Using Triacetin as a Substrate
- 1 January 1982
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 15 (13) , 1119-1129
- https://doi.org/10.1080/00032718208064425
Abstract
A conductimetric method has been applied to measure lipase activity. When using triacetin as a substrate, a linear relationship between initial rate and enzyme concentration is demonstrated up to 600 U in the cell (4 ml). Kinetic parameters of triacetin hydrolysis have been derived from conductimetric data, in the concentration range of solubility of the substrate. The limiting parameters (temperature, choice of buffer, substrate) are discussed in the last part.Keywords
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