Discovering New Natural Products Directly from Crude Extracts by HPLC-SPE-NMR: Chinane Diterpenes in Harpagophytum procumbens
- 16 February 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Natural Products
- Vol. 69 (4) , 527-530
- https://doi.org/10.1021/np050504g
Abstract
HPLC-SPE-NMR experiments with crude extracts of Harpagophytum procumbens allowed the rapid identification of novel, unstable chinane-type tricyclic diterpenes (1 and 2), along with numerous other constituents. Dramatic sensitivity gains achieved with this novel hyphenated technique establish a new paradigm in natural products research: rapid and rigorous structure determination of multiple extract components, including minor components, without preparative-scale isolation.Keywords
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