Metabolism of Bovine Semen. VII. Pyruvate-Alanine Conversion

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Abstract
Conversion of pyruvate to alanine and the reverse reaction have been demonstrated in dialyzed extracts of bovine spermatozoa and of seminal plasma, and in intact washed spermatozoa. Using washed spermatozoa, substituting Ringer phosphate-glycine for Ringer phosphate, results in an increase in alanine and a decrease in pyruvate. It is suggested that the glycine-containing buffer forces the transamination reaction glycine + pyruvate alanine + glyoxylate to the right, and that reaction accounts for the reduced accumulation of lactate in diluents containing glycine.