Complete nucleotide sequence of alfalfa mosaic virus RNA 1

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Abstract
Double-stranded cDNA of alfalfa mosaic virus (AIMV)RNA 1 has been cloned and sequenced. From clones with overlapping inserts, and other sequence data, the complete primary sequence of the 3644 nucleotides of RNA 1 was deduced: a long open reading frame for a protein of Mr 125,685 is flanked by a 5′-terminal sequence of 100 nucleotides and a 3′ noncoding region of 163 nucleotides,including the sequence of 145 nucleotides the three genomic RNAs of AIMV have in common.The two UGA-termination codons halfway RNA 1, that were postulated by Van Tol et al.(FEBS Lett. 118, 67–71,1980) to account for partial translation of RNA 1 in vitro into Mr 58,000 and Mr 62,000 proteins, were not found in the reading frame of the Mr 125,685 protein.