Transformation of Filamentous Fungi with Plasmid DNA by Electroporation
- 1 January 1989
- journal article
- research article
- Published by Scientific Societies in Phytopathology®
- Vol. 79 (8) , 844-847
- https://doi.org/10.1094/phyto-79-844
Abstract
We investigated the use of high-voltage electric pulse to transform Fusarium solani and Aspergillus nidulans with DNA. This method involved exposure of fungal protoplasts, in the presence of vector DNA, to a high-amplitude exponential-decay electric pulse of short duration (up to 123 msec) delivered from a Gene Pulser apparatus. The effects of pulse amplitude and duration on transformation frequency and cell viability were evaluated. The optimal transformation is rapid, simple to perform, and avoids the use of toxic chemicals. It may also provide an alternative method of introducing foreign DNA into fungi that cannot be transformed by traditional methods.This publication has 15 references indexed in Scilit:
- Comparative characteristics of a kievitone hydratase-producing isolate of Fusarium solani f. sp. phaseoli and of two strains deficient in this enzymePhysiological and Molecular Plant Pathology, 1987
- Stable transformation of tobacco by electroporation: evidence for plasmid concatenation.Proceedings of the National Academy of Sciences, 1986
- Rapid identification of mutation-complementing restriction fragments from Aspergillus nidulans cosmidsExperimental Mycology, 1985
- High Yields of DNA-Transfer into Mouse L-Cells by ElectropermeabilizationZeitschrift für Naturforschung C, 1985
- A cosmid for selecting genes by complementation in Aspergillus nidulans : Selection of the developmentally regulated yA locusProceedings of the National Academy of Sciences, 1985
- Transformation of Aspergillus nidulans by using a trpC plasmid.Proceedings of the National Academy of Sciences, 1984
- Isolation of DNA from filamentous fungi and separation into nuclear, mitochondrial, ribosomal, and plasmid componentsAnalytical Biochemistry, 1983
- Cloning and characterization of the ornithine carbamoyltransferase gene from Aspergillus nidulansGene, 1983
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry, 1983
- Efficient transformation of Neurospora crassa by utilizing hybrid plasmid DNAProceedings of the National Academy of Sciences, 1979