Substrates of DNA polymerases with planar conformation of sugar: model of substrate transition state?
- 1 July 1998
- journal article
- research article
- Published by Taylor & Francis in Nucleosides and Nucleotides
- Vol. 17 (7) , 1153-1162
- https://doi.org/10.1080/07328319808004228
Abstract
Several years ago, we published an hypothesis concerning conformation of the glycone moiety of different substrates in active centers of several DNA metabolizing enzymes (Nucleosides & Nucleotides 1993, 12, 649–670). This hypothesis prompted us to further study the subtle conformational changes on substrates of DNA polymerases. Data collected in our, as well as other laboratories, have been analyzed, and models of active centers of different DNA polymerases are discussed below. Based on the model of substrate requirements, we now can divide DNA polymerases into two distinguished classes.Keywords
This publication has 23 references indexed in Scilit:
- Modified nucleoside 5'‐triphosphates containing 2',3'‐fused threemembered rings as substrates for different DNA polymerasesFEBS Letters, 1993
- Possibility for the Existence of a General Conformational Motif in the Active Sites of Enzymes Which are Involved in Nucleic Acids MetabolismNucleosides and Nucleotides, 1993
- A Comparison of the Conformations of the 5′-Triphosphates of Zidovudine (AZT) and Thymidine Bound to HIV-1 Reverse TranscriptaseBiochemical and Biophysical Research Communications, 1993
- Acyclic 2′, 3′-Dideoxy-2′,3′-didehydronucleoside 5′-Triphosphates as Termination Substrates of Broad Set of DNA PolymerasesNucleosides and Nucleotides, 1993
- Structural studies of the anti-HIV agent 2′,3′-didehydro-2′,3′-dideoxythymidine (D4T)Biochemical and Biophysical Research Communications, 1991
- 1-(2,3-Dideoxy-.beta.-D-glycero-pent-2-enofuranosyl)thymine. A highly potent and selective anti-HIV agentJournal of Medicinal Chemistry, 1989
- Inhibition of HIV-associated reverse transcriptase by sugar-modified derivatives of thymidine 5′-triphosphate in comparison to cellular DNA polymerases α and βBiochemical and Biophysical Research Communications, 1987
- Properties of 2′,3′‐dideoxy‐2′,3′‐dehydrothymidine 5′‐triphosphate in terminating DNA synthesis catalyzed by several different DNA polymerasesFEBS Letters, 1987
- NMR studies of the conformations and interactions of substrates and ribonucleotide templates bound to the large fragment of DNA polymerase IBiochemistry, 1986
- Nuclear Overhauser effect studies of the conformations and binding site environments of deoxynucleoside triphosphate substrates bound to DNA polymerase I and its large fragmentBiochemistry, 1985