Stabilization of Nitrate Reductase in Maize Roots by Chymostatin

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Abstract
Nitrate reductase (NR) in maize (Zea mays cv W64A .times. W182E) roots has been stabilized in vitro by the addition of chymostatin to extraction buffer. Contrary to previous observations, levels of NR were higher in the mature root than in root tip sections when chymostatin was included in the extraction buffer. Two forms of NR were identified, an NADH monospecific NR found mainly in the 1cm root tip and an NAD(P)H bispecific HR found predominantly in mature regions of the root. During the first 10 days of seeding growth, NR activity in the root ranged from 50 to 80% of the activities found in the leaf (a maximum of 2.4 micromoles NO2- produced per hour per gram fresh weight was measured at 4 days).