Monoclonal Antibody which Recognizes a Common Antigenic Determinant on Intermediate Filament Proteins, Actin, and Myosin
- 1 January 1983
- journal article
- research article
- Published by Mary Ann Liebert Inc in Hybridoma
- Vol. 2 (3) , 275-285
- https://doi.org/10.1089/hyb.1983.2.275
Abstract
A monoclonal antibody is described which reacts with the intermediate filament proteins vimentin, desmin, keratins, actin and myosin. This is the 1st report of an epitope common to intermediate filament proteins and myosin. X1, the wide-spectrum monoclonal antibody in question, was isolated in the course of screening monoclonal antibodies to chicken thymocytes. Cross-reactivities were investigated by immunofluorescence on various types of cultured cells and sectioned tissues, ELISA [enzyme-linked immunosorbent assay] with a panel of purified antigens, immunoprecipitation, immunodot tests and immunoblotting.This publication has 31 references indexed in Scilit:
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