Drug‐induced hypomethylation of a posttranscriptionally silenced transgene locus of tobacco leads to partial release of silencing
- 2 February 2000
- journal article
- Published by Wiley in FEBS Letters
- Vol. 467 (1) , 47-51
- https://doi.org/10.1016/s0014-5793(00)01077-2
Abstract
The effect of DNA methylation upon posttranscriptional gene silencing (PTGS) has been investigated in transgenic tobacco lines showing PTGS and methylation of the neomycin phosphotransferase II (nptII) reporter genes. Application of the hypomethylation drugs dihydroxypropyladenine or 5-azacytidine resulted in approximately 30% reduced methylation of cytosines located in a non-symmetrical context in the 3′ untranslated region of the nptII transgenes. The hypomethylation was accompanied by up to 12-fold increase in NPTII protein levels, suggesting that methylation of non-symmetrical motifs may account for an increased degree of PTGS. Models for the possible role of DNA methylation in PTGS are discussed.Keywords
This publication has 36 references indexed in Scilit:
- Cross‐talk between posttranscriptionally silenced neomycin phosphotransferase II transgenesFEBS Letters, 2000
- Post‐transcriptional gene silencing of chalcone synthase in transgenic petunias, cytosine methylation and epigenetic variationThe Plant Journal, 1999
- Mapping of 5-methylcytosine residues in Nicotiana tabacum 5S rRNA genes by genomic sequencingMolecular Genetics and Genomics, 1998
- Post‐transcriptional silencing of a neomycin phosphotransferase II transgene correlates with the accumulation of unproductive RNAs and with increased cytosine methylation of 3′ flanking regionsThe Plant Journal, 1997
- Suppression of Virus Accumulation in Transgenic Plants Exhibiting Silencing of Nuclear Genes.Plant Cell, 1996
- Suppression of Virus Accumulation in Transgenic Plants Exhibiting Silencing of Nuclear GenesPlant Cell, 1996
- Genetic and biochemical dissection of transgenic RNA-mediated virus resistance.Plant Cell, 1996
- Sequence‐specific hypomethylation of the tobacco genome induced with dihydroxypropyladenine, ethionine and 5‐azacytidineFEBS Letters, 1994
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Aliphatic analogues of nucleosides, nucleotides, and oligonucleotidesCollection of Czechoslovak Chemical Communications, 1975