Development of a broad-host-range sacB-based vector for unmarked allelic exchange
Open Access
- 1 January 2008
- journal article
- Published by Springer Nature in BMC Research Notes
- Vol. 1 (1) , 1
- https://doi.org/10.1186/1756-0500-1-1
Abstract
Although genome sequences are available for an ever-increasing number of bacterial species, the availability of facile genetic tools for physiological analysis have generally lagged substantially behind traditional genetic models.Keywords
This publication has 15 references indexed in Scilit:
- Improvement of pCVD442, a suicide plasmid for gene allele exchange in bacteriaPlasmid, 2004
- Gene replacement without selection: regulated suppression of amber mutations in Escherichia coliGene, 2003
- Broad-Host-Range cre-lox System for Antibiotic Marker Recycling in Gram-Negative BacteriaBioTechniques, 2002
- C 1 Transfer Enzymes and Coenzymes Linking Methylotrophic Bacteria and Methanogenic ArchaeaScience, 1998
- A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutantsGene, 1998
- Precise Deletions in Large Bacterial Genomes by Vector-mediated Excision (VEX)Journal of Molecular Biology, 1993
- Cloning, mutagenesis, and physiological effect of a hydroxypyruvate reductase gene from Methylobacterium extorquens AM1Journal of Bacteriology, 1992
- New method for generating deletions and gene replacements in Escherichia coliJournal of Bacteriology, 1989
- Isolation and complementation analysis of 10 methanol oxidation mutant classes and identification of the methanol dehydrogenase structural gene of Methylobacterium sp. strain AM1Journal of Bacteriology, 1986
- Microbial growth on C1 compounds. 1. Isolation and characterization of Pseudomonas AM 1Biochemical Journal, 1961