Induction of a cyclic AMP-responsive gene in living cells requires the nuclear factor CREB.
Open Access
- 1 March 1991
- journal article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 11 (3) , 1759-1764
- https://doi.org/10.1128/mcb.11.3.1759
Abstract
We constructed cell lines containing various enhancer elements cloned upstream from a marker gene. By microinjection of specific antibodies directed against transcriptional activator proteins into these cell lines, we have developed a functional assay for factors which regulate the activity of target promoters. Here we show that microinjection of a highly specific antibody to the cyclic AMP enhancer element-binding (CREB) protein diminishes gene expression in response to cyclic AMP in living fibroblasts.Keywords
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