Site-directed cleavage of RNA
Open Access
- 11 June 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (11) , 4403-4415
- https://doi.org/10.1093/nar/15.11.4403
Abstract
Using complementary chimeric oligonucleotides containing deoxyribonucleotides and 2′-O-methylribonucleotides (1), enzymatically synthesized RNA (90 mer) were cleaved at a single site with Eschericia coli RNaseH, either at a hairpin loop or at a stem region. Especially, site-specific cleavage occured in even the target region being enclosed within a stable, base-paired stem. The method is proved to be generally applicable to RNA containing secondary structures.Keywords
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