THE UV ACTION SPECTRA FOR THE CLONE-FORMING ABILITY OF CULTURED HUMAN MELANOCYTES AND KERATINOCYTES
- 1 April 1994
- journal article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 59 (4) , 430-436
- https://doi.org/10.1111/j.1751-1097.1994.tb05060.x
Abstract
Melanocytes (skin type 2) and keratinocytes were irradiated with UV light of 254, 297, 302, 312 and 365 nm and the survival was measured. Clone-forming ability was chosen as the parameter for cell survival. Melanocytes were found to be less sensitive to UV light than keratinocytes (a difference of a factor 1.22-1.92 for the UV-C and UV-B wavelengths (254, 297, 301 and 312 nm) and a factor 6.71 for the UV-A wavelength (365 nm). Because melanin does not appear to protect against the induction of pyrimidine dimers the difference between melanocytes and keratinocytes in the UV-C and UV-B region could not be explained by the presence of melanin in the melanocytes. The relatively small difference can be explained by the longer cell cycle of melanocytes, which provides more time for the melanocytes to repair UV damage. In the UV-A region the difference between melanocytes and keratinocytes was much larger, suggesting that besides the longer cell cycle some additional factors must be involved in protection against UV-A light.Keywords
This publication has 20 references indexed in Scilit:
- Cultured Human Melanocytes from Black and White Donors Have Different Sunlight and Ultraviolet A Radiation SensitivitiesJournal of Investigative Dermatology, 1992
- Melanoma mortality and exposure to ultraviolet radiation: An empirical relationshipEnvironment International, 1991
- Pyrimidine Dimer Induction and Repair in Cultured Human Skin Keratinocytes or Melanocytes After Irradiation with Monochromatic Ultraviolet RadiationJournal of Investigative Dermatology, 1990
- The wavelength dependence of u.v.-induced pyrimidine dimer formation, cell killing and mutation induction in human diploid skin fibroblastsCarcinogenesis: Integrative Cancer Research, 1986
- Human Melanocytes Cultured from Nevi and MelanomasJournal of Investigative Dermatology, 1986
- Action spectra for killing and mutation of Chinese hamster cells exposed to mid- and near-ultraviolet monochromatic lightMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1984
- Selective proliferation of normal human melanocytes in vitro in the presence of phorbol ester and cholera toxin.Proceedings of the National Academy of Sciences, 1982
- Calcium regulation of growth and differentiation of mouse epidermal cells in culturePublished by Elsevier ,1980
- Isolation and Growth of Adult Human Epidermal Keratinocytes in Cell CultureJournal of Investigative Dermatology, 1978
- Formation of a keratinizing epithelium in culture by a cloned cell line derived from a teratomaCell, 1975