Simultaneous High-Performance Liquid Chromatographic Determination of Amprolium, Ethopabate, Sulfaquinoxaline and N4-Acetylsulfaquinoxaline in Chicken Tissues

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Abstract
A reversed-phase high-performance liquid chromatographic method is described for the quantitative simultaneous residue determination of amprolium with fluorometric detection using post-column reaction, and ethopabate, sulfaquinoxaline and its major metabolite, N4-acetylsulfaquinoxaline with UV detection, in chicken muscle, liver, kidney, skin and plasma. Average recoveries from chicken tissues fortified with 0.1 μg/g of the four compounds tested were ranged from 81.0 to 103.8 % for individual compounds from individual tissues. Coefficients of variation were ranged from 1.1 to 8.6 %. Detection limits were 0.002–0.004 μg/g for each compound. The applicability of this method was demonstrated by determining concentrations of the four compounds in tissues from chickens administered with the three parent compounds.