Search for the female parent in the genesis of Brasscia napus by chloroplast DNA restriction patterns.

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Abstract
Chloroplast DNA (ctDNA) from Brassica spp. were analyzed to identify the female parent of B. napus, which is the amphidiploid after the interspecific hybridization between B. oleracea and B. campestris. The putative parents and their reciprocal hybrids were subjected to ctDNA analysis by Bam HI restriction patterns. ctDNA from each of the reciprocal hybrids was identical to that from the female parent of the hybrids, showing the maternal inheritance of ctDNA in these particular crosses. B. napus ctDNA was compared with the putative parental ctDNA by Bam HI and Eco RI restriction patterns. B. napus ctDNA was indistinguishable with B. oleracea ctDNA and different from B. campestris ctDNA. B. oleracea was apparently the female parent of B. napus, although it seems very rare to obtain the hybrids by cross between .female. B. oleracea and .male. B. campestris in nature.